Example of Volunteer Data

Marshwood High School, Eliot, Maine. Piscataqua River Site, 1996.

I. Data Set & Research Descriptors

1. Principal investigator & contact person
Joyce Tugel, Chemistry Teacher
Marshwood High School
204 Dow Highway
Eliot, ME 03903
(207) 439-5600

email: jtugel@nh.ultranet.com

2. Entry verification
As tests are conducted by student volunteers, the information is recorded on a data sheet provided by the Great Bay Watch (NH) Water Quality Monitoring Program. Students then enter the data into the "Alice" software database using a Power Mac 5200. Data files are cross-checked by another student and the principal investigator prior to submission to the Wells (ME) Reserve.

3. Experimental design
The Marshwood High School Water Monitoring Program was founded four years ago as a part of the Great Bay Watch Citizen's Water Quality Monitoring Program. Over 15 Great Bay Watch sites have been established in the Great Bay Estuary, and samples are collected from April through November. The objective of the Great Bay Watch is to establish a long-term database that will provide information about the state of the estuary and to increase knowledge and interest among its members and constituents about the importance of conserving it. The Marshwood High site is located on the Piscataqua River, and provides an opportunity for high school students to volunteer in a program with "real science" applications.

4. Research methods
Water samples are collected from the Piscataqua River site using a 5 gallon bucket with a clamped vinyl decanting tube attached. The samples are analyzed for a) temperature; b) salinity; c) pH; d) dissolved oxygen. Direct measurements of e) air temp; f) turbidity; and g) water depth are also taken. A sample is also collected for h) fecal coliform bacteria. All samples are collected and analyzed using procedures in compliance with the Great Bay Watch QA/QC protocol. Detailed descriptions of the parameters and procedures are avaliable in the Great Bay Watch training manual; a summary of the procedures is included in this document. In addition to conducting the appropriate calibrations on the day of sampling, all equipment is calibrated at the beginning and midway through the sampling season by the Great Bay Watch technical advisory team.

The directors of the Great Bay Watch volunteer monitoring program conducted a technical training session at Kingman Farm, the Sea Grant/Cooperative Extension laboratory facility, in March of 1996. The high school students and teachers participating in the project spent six hours performing the analyses, and all test results compared to samples of known concentration within the accepted QA/QC limits. The teachers attend two additional quality control sessions each year, and split samples are collected by the Great Bay Watch QA/QC team and analyzed at the Piscataqua River site twice each year. The teachers are present at every sampling session, and supervise the students' protocol.

a) Water temperature is measured in degrees Celsius with an armored thermometer.
b) Salinity is determined with a hydrometer, which measures the density of the water. This is converted to salinity by measuring the temperature and using tables that relate the two measurements. The salinity is extrapolated in units of parts per thousand.
c) pH is measured with a pocket meter, which has first been calibrated with a pH 7.0 buffer solution. The meter is rinsed twice with tap water before and after the buffer calibration and once with sample water before the sample analysis.
d) Dissolved oxygen is measured by Winkler titration. The protocol outlined in the Great Bay Watch manual is similar to the methods used with a LaMotte DO kit, but the samples are collected in 250 mL BOD glass bottles, and 100 mL subsamples are titrated with manganese sulfate, iodide-azide, starch, sulfamic acid, and sodium thiosulfate. A minimum of two titrations are conducted on each sample, agreeing within 0.5 mL.
e) Air temperature is measured in degrees Celsius with an air thermometer.
f) Turbidity is measured with a Secchi disk, with lines marked every 5 cm. The disk is lowered until it just goes out of sight, raised until it just reappears, and the two measurements are averaged.
g) Water depth is measured with the Secchi disk by lowering the disk until it touches bottom.
h) Water samples for fecal coliform bacteria counts are collected in sterile Whirlpak bags. Tongs are used to lower the bags approximately 12 inches below the surface, and samples are refrigerated until analyzed later that day. Trained monitors conduct the membrane filtration process, with 100 mL water samples filtered and incubated with Gelman MFC broth at 44.5 C for 24 hours. Negative controls are run at the beginning and end of each filtration session.

5. Site location and character
The Great Bay Estuary is one of two estuaries on the short coastline of New Hampshire. It is a complex embayment composed of the Piscataqua River, Little Bay, and Great Bay. It drains a watershed of 930 square miles, one-third of which is in Maine. Eight rivers flow into the estuary, and one of them, the Piscataqua, is part of the boundary between Maine and New Hampshire.

The Great Bay Estuary interacts with the Gulf of Maine at the mouth of the Piscataqua River which flows through the industrialized city of Portsmouth, New Hampshire. Approximately 14,500 years ago, the last glacier to cover the region retreated. Rising sea waters flowed into the basin that is now Great Bay proper, which was formed while the earth's crust was still compressed by the weight of the melting glacier. The waters drowned several river valleys and formed the Great Bay Estuary. Today, strong, tidally dominated and wind-driven currents control circulation. Sediments are resuspended and nutrients are widely distributed, thereby influencing primary productivity. Important habitats in the estuary include its waters, shellfish beds, subtidal eelgrass beds, mudflats, saltmarshes, channel bottoms and rocky intertidal areas.

The rapidly increasing population has heightened demands on the estuary in terms of shoreline development and recreational pursuits such as boating, fishing, hunting, and birdwatching.

Although it is known as one of the more pristine estuary systems on the Northeast coast, Great Bay has been identified as having potential problems with water quality. There are sewage treatment plants on most of the estuary's major rivers. Although most have been upgraded to secondary treatment levels, high bacterial counts occur in some parts of the estuary.

Today, 66% of the estuary's shellfish beds have been closed due to bacterial pollution. The US Department of Agriculture has identified the Great Bay as an estuary at risk. The Great Bay receives relatively low levels of both nitrogen and phosphorus compared to similar estuaries. However, more shoreline development carries with it the risk of increased nutrients entering the estuary.

Another source of potential degradation is related to petroleum products. The Port of Portsmouth is an increasingly busy shipping port, with 91% of its commercial shipping carrying petroleum products to and from the holding tank farms on the New Hampshire shores of the Piscataqua River. A tanker spilled nearly 1,000 gallons of oil into the Piscataqua River July 1, 1996 when the moorings that attached the vessel to a dock at Schiller Station Separated. While most of the oil was contained to the Piscataqua River, some seeped into other areas of the Great Bay Estuary.
Additionally, several sites with potential for toxic substance pollution exist on the shores of the estuary. They include old tanning and bleach factories, the former Pease Air Force Base with its 14 Super Fund sites, and the Portsmouth Naval Shipyard.

The Marshwood High site is located in the Lower Piscataqua River near the Patten Yacht Yard, Inc., in South Eliot, ME (43 07' N Latitude, 70 47' W Longitude). This site is relatively close to the ocean, so its cool temperatures (low tide mean = 14.0 C, high tide mean = 12.5 C), high salinities (low tide mean = 27.6 ppt, high tide mean = 30.9 ppt), and relatively clear water (high tide mean secchi depth = 408 cm) are quite characteristic of its location. Dissolved oxygen levels are good at this site, with high and low tide saturation values averaging 96% and 95.4%, respectively. Fecal coliform levels tend to be low, with high and low tide geometric means of 3.2 and 4.9, respectively. (ref: The Great Bay Watch Five Year Report, 1990-1994; UNHMP-AR-SG-95-1)

6. Data collection period
Samples were collected from April 18, 1996 through November 6, 1996. The sampling occured approximately every other week, and on each date, samples were taken at both low and high tides.

7. Associated researchers and projects
The Maine Partners in Monitoring and New Hampshire Great Bay Watch Programs receive our data. In return, we have access to all the PIM and GBW datasets from each of the sampling years. We also submit our data to the Eliot and South Berwick Conservation Commissions, who help support us financially.

II. Data Table Descriptors

1. The data appears in the following order (note that L = low tide, H = high tide): Date; water temp L (oC); water temp H (oC); dissolved oxygen L (ppm); dissolved oxygen H (ppm); salinity L (ppt); salinity H (ppt); saturation L (%); saturation H (%); pH L; pH H; fecal coliform L (cfu/100mL); fecal coliform H (cfu/100mL); transparency (turbidity) L (cm); transparency (turbidity) H (cm); depth L (cm); depth H (cm); air temp L (oC); air temp H (oC).

2. Remarks
April 18: Weather partly cloudy, boating activities
May 6: Weather overcast, boating activities
May 20: Weather foggy, boating activities
June 3: Weather overcast/shower, no boating activities
June 17: Weather clear, boating activities
July 1: Weather partly cloudy, boat maintenance, fish jumping
July 15: Weather overcast, boaat maintenance
July 30: Weather partly cloudy, boating activities
Aug 14: Weather clear, boating activities
Aug 29: Weather overcast, tanks unloading, boating activites
Sept 16: Weather overcast, boating activites
Sept 30: Weather overcast, windy, no activities
Oct 15: Weather clear, windy, boating
Oct 29: Weather partly cloudy, boating, boat maintenance
Nov 6: Weather clear, boat repair, oil film

3. Explanations for missing data
Depth readings not possible due to strong currents on 5/6/96 low tide and 7/1/96, 10/15/96, and 10/29/96 high tides. A period (.) was placed in the data table for the parameters that we do not test for.